- Title
- Toll/IL-1 signaling is critical for house dust mite-specific Th1 and Th2 responses
- Creator
- Phipps, Simon; Lam, Chuan En; Mansell, Ashley; Matthaei, Klaus I.; Foster, Paul S.; Kaiko, Gerard E.; Foo, Shen Yun; Collison, Adam; Mattes, Joerg; Barry, Jessica; Davidson, Sophia; Oreo, Kevin; Smith, Lauren
- Relation
- American Journal of Respiratory and Critical Care Medicine Vol. 179, Issue 10, p. 883-893
- Publisher Link
- http://dx.doi.org/10.1164/rccm.200806-974OC
- Publisher
- American Thoracic Society
- Resource Type
- journal article
- Date
- 2009
- Description
- Rationale: One of the immunopathological features of allergic inflammation is the infiltration of helper T type 2 (Th2) cells to the site of disease. Activation of innate pattern recognition receptors such as Toll-like receptors (TLRs) plays a critical role in helper T type 1 cell differentiation, yet their contribution to the generation of Th2 responses to clinically relevant aeroallergens remains poorly defined. Objectives: To determine the requirement for TLR2, TLR4, and the Toll/ IL-1 receptor domain adaptor protein MyD88 in a murine model of allergic asthma. Methods: Wild-type and factor-deficient (⁻/⁻) mice were sensitized intranasally to the common allergen house dust mite (HDM) and challenged 2 weeks later on four consecutive days. Measurements of allergic airway inflammation, T-cell cytokine production, and airway hyperreactivity were performed 24 hours later. Measurements and Main Results: Mice deficient in MyD88 were protected from the cardinal features of allergic asthma, including granulocytic inflammation, Th2 cytokine production and airway hyperreactivity. Although HDM activated NF-kB in TLR2- or TLR4-expressing HEκ cells, only in TLR4⁻/⁻ mice was the magnitude of allergic airway inflammation and hyperreactivity attenuated. The diminished Th2 response present in MyD88⁻/⁻ and TLR4⁻/⁻ mice was associated with fewer OX40 ligand–expressing myeloid dendritic cells in the draininglymph nodes during allergic sensitization. Finally, HDM-specific IL-17 production and airway neutrophilia were attenuated in MyD88⁻/⁻ but not TLR4⁻/⁻ mice. Conclusions: Together, these data suggest that Th2-andTh17-mediated inflammation generated on inhalational HDM exposure is differentially regulated by the presence of microbial products and the activation of distinct MyD88-dependent pattern recognition receptors.
- Subject
- asthma; innate immunity; eosinophil; neutrophil
- Identifier
- http://hdl.handle.net/1959.13/806906
- Identifier
- uon:7255
- Identifier
- ISSN:1073-449X
- Language
- eng
- Reviewed
- Hits: 6751
- Visitors: 7412
- Downloads: 0
| Thumbnail | File | Description | Size | Format |
|---|